Fluorescent in situ hybridization Reagents
ABclonal Technology LIMK1 Rabbit pAb
LIM proteins, including LIM kinase-1 (LIMK1), contain LIM domains with two zinc fingers, likely mediating protein-protein interactions. LIMK1, part of a subfamily with two LIM motifs and a kinase domain, is a serine/threonine kinase that regulates actin polymerization by phosphorylating and inactivating cofilin. It is widely expressed during development and plays a role in cytoskeletal structure, axon growth, and brain development. LIMK1 hemizygosity is linked to impaired visuospatial cognition in Williams syndrome. Alternative splicing produces multiple isoforms of this protein.
ABclonal Technology VEGFA Rabbit pAb
This gene, part of the PDGF/VEGF family, encodes a heparin-binding, disulfide-linked homodimeric growth factor essential for angiogenesis. It promotes vascular endothelial cell proliferation and migration, with gene disruption in mice causing abnormal embryonic vessel formation. Upregulated in many tumors, its expression correlates with tumor progression. High protein levels are seen in POEMS syndrome and associated with diabetes-related microvascular issues and atherosclerosis. Multiple isoforms arise via alternative splicing, non-AUG initiation, and stop codon readthrough, with one extended isoform being antiangiogenic. Some AUG-derived isoforms are regulated by a uORF within an IRES. VEGF levels increase in SARS-CoV-2 infection, promoting inflammation via inflammatory cell recruitment and upregulation of angiopoietin II, creating a feedback loop that amplifies cytokine release.
Cayman Chemical THUNDRHumn IL10 TRFRET 2400Wel
A sandwich TR-FRET immunoassay for the quantitative measurement of human IL-10 (CSIF) in cell culture supernatants
ABclonal Technology CDKN2A/p16INK4a Rabbit pAb
This gene produces several transcript variants with different first exons. At least three spliced variants encode distinct proteins, two of which are CDK4 kinase inhibitors. The third variant includes an alternate first exon and open reading frame (ARF), encoding a protein that stabilizes the tumor suppressor p53. This ARF product interacts with and sequesters MDM2, an E3 ubiquitin ligase that degrades p53. Despite structural differences, both the CDK inhibitor isoforms and ARF product share a common role in regulating G1 progression of the cell cycle through CDK4 and p53. This gene is frequently mutated or deleted in many tumors, making it an important tumor suppressor.
ABclonal Technology CDKN2A/p16INK4a Rabbit pAb
This gene produces several transcript variants with different first exons. At least three spliced variants encode distinct proteins, two of which are CDK4 kinase inhibitors. The third variant includes an alternate first exon and open reading frame (ARF), encoding a protein that stabilizes the tumor suppressor p53. This ARF product interacts with and sequesters MDM2, an E3 ubiquitin ligase that degrades p53. Despite structural differences, both the CDK inhibitor isoforms and ARF product share a common role in regulating G1 progression of the cell cycle through CDK4 and p53. This gene is frequently mutated or deleted in many tumors, making it an important tumor suppressor.
Cayman Chemical THUNDRHumn IL10 TRFRET 4800Wel
A sandwich TR-FRET immunoassay for the quantitative measurement of human IL-10 (CSIF) in cell culture supernatants